Anti-CLM-9 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CLM-9 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CLM-9 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CLM-9 Magnetic Beads-IP Kit Product Description
The Anti-CLM-9 magnetic Beads, conjugated with Anti-CLM-9 antibody, are used for immuneprecipitation (IP) of CLM-9 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CLM-9 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CLM-9 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CLM-9 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CLM-9 / TREM4 protein (Catalog#13365-H08H)
Species Reactivity
Human CLM-9 / TREM4
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CLM-9 / TREM4 (rh CLM-9 / TREM4; Catalog#13365-H08H; AAH25395.1; Met1-Arg247). CLM-9 / TREM4 specific IgG was purified by Human CLM-9 / TREM4 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CLM-9 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CLM-9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CLM9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NEPMUCINALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TREM-4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TREM4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CLM-9 Background Information
CLM-9, also known as TREM4, is a receptor which belongs to the TREM family. The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. CLM-9 may mediate L-selectin-dependent lymphocyte rollings. It binds SELL in a calcium dependent manner. CLM-9 also binds lymphocyte which suggests that it functions in lymphocyte adhesion. The major CLM-9 transcript is expressed highly in human heart, skeletal muscle, and placenta. The mouse protein has been shown to be expressed in capillary endothelial cells. Human CLM-9 mediates the uptake of human IgA2 and mouse IgM.
Full Name
CD300 molecule-like family member g
References
Clark GJ, et al. (2009) The CD300 molecules regulate monocyte and dendritic cell functions. Immunobiology. 214(9-10):730-6. Engel K, et al. (2012) Bacterial expression of mutant argininosuccinate lyase reveals imperfect correlation of in-vitro enzyme activity with clinical phenotype in argininosuccinic aciduria. J Inherit Metab Dis. 35(1):133-40. Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics. 89(3):307-15.
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