Anti-ERP72 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ERP72 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ERP72 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ERP72 Magnetic Beads-IP Kit Product Description
The Anti-ERP72 magnetic Beads, conjugated with Anti-ERP72 antibody, are used for immuneprecipitation (IP) of ERP72 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ERP72 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ERP72 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ERP72 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ERP72 / PDIA4 protein (Catalog#13333-H08H)
Species Reactivity
Human ERP72 / PDIA4
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ERP72 / PDIA4 (rh ERP72 / PDIA4; Catalog#13333-H08H; P13667; Met1-Thr641). ERP72 / PDIA4 specific IgG was purified by Human ERP72 / PDIA4 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ERP72 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ERp-72ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ERP70ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ERP72ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ERP72 Background Information
ERP72, also known as PDIA4, is an endoplasmic reticulum luminal protein which belongs to the protein disulfide isomerase family. ERP72 is a stress protein and participates in the catalysis of protein-S-S-bond rearrangement. Both of PDIA4 and PDIA3 function as proteases, protein disulfide isomerases, phospholipases or an arrangement of these. ERP72 compose part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP9B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Full Name
protein disulfide isomerase family A, member 4
References
Tsai YC, et al. (2012) Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription. Mol Cell Proteomics. 11(5):60-76. Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40. Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.
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