Anti-FKBP7 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-FKBP7 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FKBP7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FKBP7 Magnetic Beads-IP Kit Product Description
The Anti-FKBP7 magnetic Beads, conjugated with Anti-FKBP7 antibody, are used for immuneprecipitation (IP) of FKBP7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FKBP7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FKBP7 proteins are dissociated from the magnetic beads using an elution buffer. Anti-FKBP7 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human FKBP7 / Rotamase protein (Catalog#13250-H08H)
Species Reactivity
Human FKBP7 / Rotamase
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human FKBP7 / Rotamase (rh FKBP7 / Rotamase; Catalog#13250-H08H; Q9Y680-2; Met1-Gln218). FKBP7 / Rotamase specific IgG was purified by Human FKBP7 / Rotamase affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FKBP7 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-FKBP23ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PPIaseALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FKBP7 Background Information
PPIase is a member of the immunophilin protein family. It also belongs to the cyclophilin-type PPIase family, PPIL3 subfamily. PPIase contains 1 PPIase cyclophilin-type domain. Members of the immunophilin protein family play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It has a very high substrate specificity for the four-residue peptide Ala-Ala-Pro-Phe only when the proline peptide bond is in the trans state. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.
Full Name
FK506 binding protein 7
References
1. Shor B, et al. (2008) A new pharmacologic action of CCI-779 involves FKBP12-independent inhibition of mTOR kinase activity and profound repression of global protein synthesis. Cancer Res. 68(8):2934-43. 2. Talmud PJ, et al. (2009) Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Am J Hum Genet. 85(5):628-42. 3. Deleersnijder A, et al. (2011) Comparative analysis of different peptidyl-prolyl isomerases reveals FK506-binding protein 12 as the most potent enhancer of alpha-synuclein aggregation. J Biol Chem. 286(30):26687-701.
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