Anti-FDPS Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-FDPS Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FDPS magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FDPS Magnetic Beads-IP Kit Product Description
The Anti-FDPS magnetic Beads, conjugated with Anti-FDPS antibody, are used for immuneprecipitation (IP) of FDPS proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FDPS proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FDPS proteins are dissociated from the magnetic beads using an elution buffer. Anti-FDPS Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human FDPS / Farnesyl Diphosphate Synthase protein (Catalog#13229-H07E)
Species Reactivity
Human FDPS / Farnesyl Diphosph
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human FDPS / Farnesyl Diphosphate Synthase (rh FDPS / Farnesyl Diphosphate Synthase; Catalog#13229-H07E; NP_001129294.1; Met1-Lys353). FDPS / Farnesyl Diphosphate Synthase specific IgG was purified by Human FDPS / Farnesyl Diphosphate Synthase affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FDPS Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-FPPSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FPSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FDPS Background Information
Z-farnesyl diphosphate synthase (FDPS) is an enzyme belonging to the family of transferases, specifically those transferring aryl or alkyl groups other than methyl groups. Z-farnesyl diphosphate synthase (FDPS) functions as key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate, a precurcor for several classes of essential metabolites. FDPS catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Functions of FDPS may be inactivated by interferon-induced RSAD2. This inactivation may result of disruption of lipid rafts at the plasma membrane, and thus have an antiviral effect since many enveloped viruses need lipid rafts to bud efficiently out of the cell.
Full Name
farnesyl diphosphate synthase
References
Pjetursson BE, et al. (2007) Comparison of survival and complication rates of tooth-supported fixed dental prostheses (FDPs) and implant-supported FDPs and single crowns (SCs). Clin Oral Implants Res. 3:97-113. Eschbach S, et al. (2009) Clinical evaluation of all-ceramic posterior three-unit FDPs made of In-Ceram Zirconia. Int J Prosthodont. 22(5):490-2. Moshage HJ, et al. (1990) Differential effects of endotoxin and fibrinogen degradation products (FDPS) on liver synthesis of fibrinogen and albumin: evidence for the involvement of a novel monokine in the stimulation of fibrinogen synthesis induced by FDPS. Int J Biochem. 22(12): 1393-400.
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