Anti-SerpinB3 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SerpinB3 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SerpinB3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SerpinB3 Magnetic Beads-IP Kit Product Description
The Anti-SerpinB3 magnetic Beads, conjugated with Anti-SerpinB3 antibody, are used for immuneprecipitation (IP) of SerpinB3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SerpinB3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SerpinB3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SerpinB3 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SERPINB3 / SCCA-1 protein (Catalog#13218-H07B)
Species Reactivity
Human SERPINB3 / SCCA-1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SERPINB3 / SCCA-1 (rh SERPINB3 / SCCA-1; Catalog#13218-H07B; AAB20405.1; Asn2-Pro390). SERPINB3 / SCCA-1 specific IgG was purified by Human SERPINB3 / SCCA-1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SerpinB3 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HsT1196ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCCA-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCCA-PDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCCA1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-T4-AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SerpinB3 Background Information
SERPINB3, also known as SCCA-1, belongs to the serpin family. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. SERPINB3 is expressed in some hepatocellular carcinoma (at protein level). Its expression is closely related to cellular differentiation in both normal and malignant squamous cells. It seems to also be secreted in plasma by cancerous cells but at a low level. SERPINB3 significantly attenuates apoptosis by contrasting cytochrome c release from the mitochondria and by antichemotactic effect for NK cells. It may act as a protease inhibitor to modulate the host immune response against tumor cells and may be involved in the malignant behavior of squamous cell carcinoma cells.
Full Name
serpin peptidase inhibitor, clade B (ovalbumin), member 3
References
Schneider SS, et al. (1995) A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene. Proc Natl Acad Sci. 92(8):3147-51. Suminami Y, et al.. (1992) Squamous cell carcinoma antigen is a new member of the serine protease inhibitors. Biochem Biophys Res Commun. 181(1):51-8. Mino-Miyagawa N, et al. (1990) Tumor-antigen 4. Its immunohistochemical distribution and tissue and serum concentrations in squamous cell carcinoma of the lung and esophagus. Cancer. 66(7):1505-12.
新浪微博
腾讯微博
QQ空间
客服
