Anti-PHOSPHO1 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-PHOSPHO1 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PHOSPHO1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PHOSPHO1 Magnetic Beads-IP Kit Product Description
The Anti-PHOSPHO1 magnetic Beads, conjugated with Anti-PHOSPHO1 antibody, are used for immuneprecipitation (IP) of PHOSPHO1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PHOSPHO1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PHOSPHO1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PHOSPHO1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PHOSPHO1 protein (Catalog#13187-H07E)
Species Reactivity
Human PHOSPHO1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PHOSPHO1 (rh PHOSPHO1; Catalog#13187-H07E; Q8TCT1-1; Met1-Cys267). PHOSPHO1 specific IgG was purified by Human PHOSPHO1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
PHOSPHO1 Background Information
Full Name
phosphatase, orphan 1
References
Stewart AJ, et al. (2003) Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily. Protein Eng. 16(12): 889-95. Camps M, et al. (2000) Dual specificity phosphatases: a gene family for control of MAP kinase function. FASEB J. 14(1): 6-16. Barford D. (1996) Molecular mechanisms of the protein serine/threonine phosphatases. Trends Biochem Sci. 21(11): 407-12.