Anti-CCDC134 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CCDC134 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CCDC134 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CCDC134 Magnetic Beads-IP Kit Product Description
The Anti-CCDC134 magnetic Beads, conjugated with Anti-CCDC134 antibody, are used for immuneprecipitation (IP) of CCDC134 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CCDC134 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CCDC134 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CCDC134 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CCDC134 protein (Catalog#13168-H08H)
Species Reactivity
Human CCDC134
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CCDC134 (rh CCDC134; Catalog#13168-H08H; Q9H6E4; Met1-Leu229). CCDC134 specific IgG was purified by Human CCDC134 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
CCDC134 Background Information
Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.
Full Name
coiled-coil domain containing 134
References
Huang J, et al. (2007) CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cellular and Molecular Life Sciences. 65(2): 338-49. Kim S, et al. (2011) Genome-wide association study of CSF biomarkers Abeta1-42, t-tau, and p-tau181p in the ADNI cohort. Neurology. 76(1): 69-79.
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