Anti-AK2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-AK2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-AK2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-AK2 Magnetic Beads-IP Kit Product Description
The Anti-AK2 magnetic Beads, conjugated with Anti-AK2 antibody, are used for immuneprecipitation (IP) of AK2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing AK2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound AK2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-AK2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human AK2 protein (Catalog#13146-H20B)
Species Reactivity
Human AK2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human AK2 (rh AK2; Catalog#13146-H20B; P54819-1; Met 1-Ile 239). AK2 specific IgG was purified by Human AK2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-AK2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ADK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
AK2 Background Information
Adenylate kinase 2 (AK2) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Adenylate kinase2 (AK2) is expressed in mitochondrial intermembrane space. It may play a role in apoptosis. It has been demonstrated that in apoptotic cells AK2 was translocated into the cytosol concomitantly with cytochronme C. Mutations in this gene are the cause of reticular dysgenesis. These mutations result in absent or strongly decreased protein expression. It has been also established that AK2 is specifically expressed in the stria vascularis region of the inner ear, which provides an explanation of the sensorineural deafness in these individuals.
Full Name
adenylate kinase 2
References
Lagresle-Peyrou C, et al. (2008) Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nature Genetics. 41: 106-11. Bruns GA, et al. (1977) Adenylate kinase 2, a mitochondrial enzyme. Biochem Genet. 15 (5-6): 477-86. Khler C, et al. (1999) Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis. FEBS Lett. 447 (1): 10-2.
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