Anti-CD3 zeta Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD3 zeta Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD3 zeta magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD3 zeta Magnetic Beads-IP Kit Product Description
The Anti-CD3 zeta magnetic Beads, conjugated with Anti-CD3 zeta antibody, are used for immuneprecipitation (IP) of CD3 zeta proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD3 zeta proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD3 zeta proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD3 zeta Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD247 / CD3-ZETA Protein (Catalog#13142-H09E)
Species Reactivity
Human CD247 / CD3-ZETA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD247 / CD3-ZETA (rh CD247 / CD3-ZETA; Catalog#13142-H09E; P20963-1; Arg52-Arg164). CD247 / CD3-ZETA specific IgG was purified by Human CD247 / CD3-ZETA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD3 zeta Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CD3-ZETAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD3HALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD3QALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD3ZALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IMD25ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-T3ZALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TCRZALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD3 zeta Background Information
CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
References
1. Radstake TR, et al. (2010) Genome-wide association study of systemic sclerosis identifies CD247 as a new susceptibility locus. Nat Genet. 42(5):426-9. 2. Dieud P, et al. (2011) Independent replication establishes the CD247 gene as a genetic systemic sclerosis susceptibility factor. Ann Rheum Dis. 70(9):1695-6. 3. Li R, et al. (2012) Association of CD247 with systemic lupus erythematosus in Asian populations. Lupus. 21(1):75-83.
新浪微博
腾讯微博
QQ空间
客服
