Anti-Bikunin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Bikunin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Bikunin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Bikunin Magnetic Beads-IP Kit Product Description
The Anti-Bikunin magnetic Beads, conjugated with Anti-Bikunin antibody, are used for immuneprecipitation (IP) of Bikunin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Bikunin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Bikunin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Bikunin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human AMBP / Alpha 1 microglobulin protein (Catalog#13141-H08H1)
Species Reactivity
Human AMBP / Alpha 1 microglob
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human AMBP / Alpha 1 microglobulin (rh AMBP / Alpha 1 microglobulin; Catalog#13141-H08H1; NP_001624.1; Met1-Val203). AMBP / Alpha 1 microglobulin specific IgG was purified by Human AMBP / Alpha 1 microglobulin affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Bikunin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-A1MALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EDC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HCPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HI30ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IATILALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ITIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ITILALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ITILCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UTIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Bikunin Background Information
The AMBP [A1M (alpha1-microglobulin)/bikunin precursor] gene encodes two plasma glycoproteins: A1M, an immunosuppressive lipocalin, and bikunin, a member of plasma serine proteinase inhibitor family with prototypical Kunitz-type domain. Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. AMBP mRNA is strongly transcribed in liver parenchyma, pancreas, and intestine epithelium. Sites of weaker expression are the vessels of the umbilical cord, the developing vertebral bodies, and kidney. The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. However, additional sites of protein distribution were found that do not correlate to mRNA localization: alpha(1)-microglobulin was found in myocytes and bikunin in cardiac muscle, nervous system microvasculature, and connective tissue
Full Name
alpha-1-microglobulin/bikunin precursor
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