Anti-Tissue Factor Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Tissue Factor Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Tissue Factor magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Tissue Factor Magnetic Beads-IP Kit Product Description
The Anti-Tissue Factor magnetic Beads, conjugated with Anti-Tissue Factor antibody, are used for immuneprecipitation (IP) of Tissue Factor proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Tissue Factor proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Tissue Factor proteins are dissociated from the magnetic beads using an elution buffer. Anti-Tissue Factor Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Coagulation Factor III / Tissue Factor / CD142 Protein (Catalog#13133-H08H)
Species Reactivity
Human Coagulation Factor III /
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Coagulation Factor III / Tissue Factor / CD142 (rh Coagulation Factor III / Tissue Factor / CD142; Catalog#13133-H08H; P13726; Met1-Glu251). Coagulation Factor III / Tissue Factor / CD142 specific IgG was purified by Human Coagulation Factor III / Tissue Factor / CD142 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Tissue Factor Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CD142ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-coagulation factor 3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-coagulation factor IIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TFAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Tissue Factor Background Information
Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.
Full Name
coagulation factor III (thromboplastin, tissue factor)
References
Morrissey JH. (2004) Tissue factor: a key molecule in hemostatic and nonhemostatic systems. Int J Hematol. 79(2): 103-8. Milsom C, et al. (2008) Tissue factor and cancer. Pathophysiol Haemost Thromb. 36(3-4): 160-76. Kasthuri RS, et al. (2009) Role of tissue factor in cancer. J Clin Oncol. 27(29): 4834-8.