Anti-IGFBP7 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-IGFBP7 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-IGFBP7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-IGFBP7 Magnetic Beads-IP Kit Product Description
The Anti-IGFBP7 magnetic Beads, conjugated with Anti-IGFBP7 antibody, are used for immuneprecipitation (IP) of IGFBP7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IGFBP7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IGFBP7 proteins are dissociated from the magnetic beads using an elution buffer. Anti-IGFBP7 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human IGFBP7 Protein (Catalog#13100-H08H)
Species Reactivity
Human IGFBP7
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human IGFBP7 (rh IGFBP7; Catalog#13100-H08H; Q16270; Met1-Leu282).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-IGFBP7 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AGMALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FSTL2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IBP-7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IGFBP-7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IGFBP-7vALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IGFBPRP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAC25ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PSFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RAMSVPSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TAFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
IGFBP7 Background Information
Insulin like growth factor binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity and induce apoptosis in RKO and SW62 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.
Full Name
insulin-like growth factor binding protein 7
References
Oh Y, et al. (1996) Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II. J Biol Chem. 271(48): 30322-5. Wilson EM, et al. (1997) Generation and characterization of an IGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578T human breast cancer conditioned media. J Clin Endocrinol Metab. 82(4): 1301-3. Lin J, et al. (2007) Methylation patterns of IGFBP7 in colon cancer cell lines are associated with levels of gene expression. J Pathol. 212(1): 83-90.
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