Anti-SUMO1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SUMO1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SUMO1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SUMO1 Magnetic Beads-IP Kit Product Description
The Anti-SUMO1 magnetic Beads, conjugated with Anti-SUMO1 antibody, are used for immuneprecipitation (IP) of SUMO1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SUMO1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SUMO1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SUMO1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SUMO1 / SUMO-1 protein (Catalog#13095-H07E)
Species Reactivity
Human SUMO1 / SUMO-1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SUMO1 / SUMO-1 (rh SUMO1 / SUMO-1; Catalog#13095-H07E; AAH66306.1; Ser 2-Val 101). SUMO1 / SUMO-1 specific IgG was purified by Human SUMO1 / SUMO-1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SUMO1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DAP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GMP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-OFC10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PIC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SENP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SMT3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SMT3CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SMT3H3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UBL1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SUMO1 Background Information
Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.
Full Name
small ubiquitin-like modifier 1
References
Kim HJ, et al. (2011) SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2. Biochem Biophys Res Commun. 410(3): 555-62. Zuo Y, et al. (2009) Small ubiquitin-like modifier protein-specific protease 1 and prostate cancer. Asian J Androl. 11(1): 36-8. Song T, et al. (2008) SUMO1 polymorphisms are associated with non-syndromic cleft lip with or without cleft palate. Biochem Biophys Res Commun. 377(4): 1265-8. Katayama A, et al. (2007) Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: possible involvement in tumor proliferation and prognosis. Int J Oncol. 31(3): 517-24.
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