Anti-NUDT5 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-NUDT5 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-NUDT5 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-NUDT5 Magnetic Beads-IP Kit Product Description
The Anti-NUDT5 magnetic Beads, conjugated with Anti-NUDT5 antibody, are used for immuneprecipitation (IP) of NUDT5 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing NUDT5 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound NUDT5 proteins are dissociated from the magnetic beads using an elution buffer. Anti-NUDT5 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ADP-sugar Pyrophosphatase / NUDT5 protein (Catalog#13068-H07E)
Species Reactivity
Human ADP-sugar Pyrophosphatas
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ADP-sugar Pyrophosphatase / NUDT5 (rh ADP-sugar Pyrophosphatase / NUDT5; Catalog#13068-H07E; Q9UKK9; Glu2-Phe219). ADP-sugar Pyrophosphatase / NUDT5 specific IgG was purified by Human ADP-sugar Pyrophosphatase / NUDT5 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-NUDT5 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-hYSAH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-YSA1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-YSA1HALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-YSAH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
NUDT5 Background Information
ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.
Full Name
nudix (nucleoside diphosphate linked moiety X)-type motif 5
References
Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7. Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101. Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.
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