Anti-GOLPH2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-GOLPH2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GOLPH2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GOLPH2 Magnetic Beads-IP Kit Product Description
The Anti-GOLPH2 magnetic Beads, conjugated with Anti-GOLPH2 antibody, are used for immuneprecipitation (IP) of GOLPH2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GOLPH2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GOLPH2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-GOLPH2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H07E)
Species Reactivity
Human GOLM1 / GP73
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73; Catalog#13066-H07E; Q8NBJ4-1; Met 1-Leu 401). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GOLPH2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-bA379P1.3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-C9orf155ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GOLPH2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GP73ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL46ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PSEC0257ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GOLPH2 Background Information
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Full Name
golgi membrane protein 1
References
Kladney R.D., et al.,(2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65. Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270. Puri S., et al.,(2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.
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