Anti-Ube2H Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Ube2H Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Ube2H magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Ube2H Magnetic Beads-IP Kit Product Description
The Anti-Ube2H magnetic Beads, conjugated with Anti-Ube2H antibody, are used for immuneprecipitation (IP) of Ube2H proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Ube2H proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Ube2H proteins are dissociated from the magnetic beads using an elution buffer. Anti-Ube2H Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human UBE2H protein (Catalog#12892-HNCE)
Species Reactivity
Human UBE2H
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human UBE2H (rh UBE2H; Catalog#12892-HNCE; P62256; Met1-Leu183). UBE2H specific IgG was purified by Human UBE2H affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Ube2H Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-E2-20KALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GID3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UBC8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UBCHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UBCH2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Ube2H Background Information
UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Full Name
ubiquitin-conjugating enzyme E2H
References
Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317. Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.
新浪微博
腾讯微博
QQ空间
客服
