Anti-Ezrin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Ezrin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Ezrin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Ezrin Magnetic Beads-IP Kit Product Description
The Anti-Ezrin magnetic Beads, conjugated with Anti-Ezrin antibody, are used for immuneprecipitation (IP) of Ezrin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Ezrin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Ezrin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Ezrin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Ezrin / villin 2 protein (Catalog#12858-H07E)
Species Reactivity
Human Ezrin / villin 2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Ezrin / villin 2 (rh Ezrin / villin 2; Catalog#12858-H07E; P15311; Pro2-Leu586). Ezrin / villin 2 specific IgG was purified by Human Ezrin / villin 2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Ezrin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CVILALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CVLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-105ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-VIL2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Ezrin Background Information
VIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskel
References
Grnholm M, et al. (1999) Homotypic and heterotypic interaction of the neurofibromatosis 2 tumor suppressor protein merlin and the ERM protein ezrin. J Cell Sci. 112(6):895-904. Sitaraman, et al. (2002) The adenosine 2b receptor is recruited to the plasma membrane and associates with E3KARP and Ezrin upon agonist stimulation. J Biol Chem. 277(36):33188-95. Gould KL, et al. (1989) cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J. 8(13):4133-42.
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