Anti-ALDH4A1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ALDH4A1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ALDH4A1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ALDH4A1 Magnetic Beads-IP Kit Product Description
The Anti-ALDH4A1 magnetic Beads, conjugated with Anti-ALDH4A1 antibody, are used for immuneprecipitation (IP) of ALDH4A1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ALDH4A1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ALDH4A1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ALDH4A1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ALDH4A1 Protein (Catalog#12856-H20B)
Species Reactivity
Human ALDH4A1
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (rh ALDH4A1; Catalog#12856-H20B; AAH07581.1; Lys25-Gln563).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ALDH4A1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ALDH4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P5CDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P5CDhALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ALDH4A1 Background Information
ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.
Full Name
aldehyde dehydrogenase 4 family, member A1
References
Goodman SI, et al. (1974) Defective hydroxyproline metabolism in type II hyperprolinemia. Biochemical medicine. 10 (4): 329-36. Maruyama K, et al. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138 (1-2): 171-4. Vasiliou V, et al. (2005) Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family. Hum Genomics. 2 (2): 138-43.
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