Anti-ILKAP Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ILKAP Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ILKAP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ILKAP Magnetic Beads-IP Kit Product Description
The Anti-ILKAP magnetic Beads, conjugated with Anti-ILKAP antibody, are used for immuneprecipitation (IP) of ILKAP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ILKAP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ILKAP proteins are dissociated from the magnetic beads using an elution buffer. Anti-ILKAP Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ILKAP protein (Catalog#12697-H08H)
Species Reactivity
Human ILKAP
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ILKAP (rh ILKAP; Catalog#12697-H08H; Q9H0C8; Met1-His392). ILKAP specific IgG was purified by Human ILKAP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ILKAP Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ILKAP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ILKAP3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PP2C-DELTAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ILKAP Background Information
Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is cytoplasm protein which belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. ILKAP may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase ( ILK ) plays key roles in a variety of cell functions, including cell proliferation, adhesion and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.
Full Name
integrin-linked kinase-associated serine/threonine phosphatase
References
Leung-Hagesteijn C. et al., 2001, EMBO J. 20: 2160-70. Kumar,A.S. et al., 2004, Oncogene. 23 (19):3454-61. Lammers,T. et al., 2007, Crit Rev Biochem Mol Biol. 42 (6):437-61. Nakrieko,K.A. et al., 2008, Cell Cycle. 7 (14):2157-66.
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