Anti-IKB beta Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-IKB beta Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-IKB beta magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-IKB beta Magnetic Beads-IP Kit Product Description
The Anti-IKB beta magnetic Beads, conjugated with Anti-IKB beta antibody, are used for immuneprecipitation (IP) of IKB beta proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IKB beta proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IKB beta proteins are dissociated from the magnetic beads using an elution buffer. Anti-IKB beta Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human NFKBIB / IkB-beta Protein
Species Reactivity
Human NFKBIB / IkB-beta
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human NFKBIB / IkB-beta (rh NFKBIB / IkB-beta; AAH15528.1; 1M-356V). NFKBIB / IkB-beta specific IgG was purified by Human NFKBIB / IkB-beta affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-IKB beta Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-IKBBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TRIP9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
IKB beta Background Information
NF-kappaB regulation involves the inhibitor proteins NF-kappaB inhibitor beta (NFKBIB), (also known as IkappaB beta), which form complexes with NF-kappaB to sequester it in the cytoplasm. Overexpression of NFKBIB protein in IAV infected cells led to lower levels of IAV. MiR-2a could promote activation of the NFkappaB pathway and downstream targets livin and survivin by targeting NFKBIB, which potentially contributed to GC chemoresistance.
Full Name
NFKB inhibitor beta
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