Anti-Ube2L6 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Ube2L6 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Ube2L6 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Ube2L6 Magnetic Beads-IP Kit Product Description
The Anti-Ube2L6 magnetic Beads, conjugated with Anti-Ube2L6 antibody, are used for immuneprecipitation (IP) of Ube2L6 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Ube2L6 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Ube2L6 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Ube2L6 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human UBCH8 / UBE2L6 protein (Catalog#12641-H07E)
Species Reactivity
Human UBCH8 / UBE2L6
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human UBCH8 / UBE2L6 (rh UBCH8 / UBE2L6; Catalog#12641-H07E; O14933-1; Met1-Ser153). UBCH8 / UBE2L6 specific IgG was purified by Human UBCH8 / UBE2L6 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Ube2L6 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-RIG-BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UBCH8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Ube2L6 Background Information
UBCH8, also known as UBE2L6, belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so, E2s differentiate effects on downstream substrates, either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.
Full Name
ubiquitin-conjugating enzyme E2L 6
References
Moynihan TP, et al. (1999) The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 274(43):30963-8. Ardley HC, et al. (2000) Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. Cytogenet Cell Genet. 89(1-2):137-40. Zhao C, et al. (2004) The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein. Proc Natl Acad. 101(20):7578-82. Tripathi MK, et al. (2005) Down-regulation of UCRP and UBE2L6 in BRCA2 knocked-down human breast cells. Biochem Biophys Res Commun. 328(1):43-8.
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