Anti-SERPINB1 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-SERPINB1 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SERPINB1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SERPINB1 Magnetic Beads-IP Kit Product Description
The Anti-SERPINB1 magnetic Beads, conjugated with Anti-SERPINB1 antibody, are used for immuneprecipitation (IP) of SERPINB1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SERPINB1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SERPINB1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SERPINB1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SerpinB1 / ELANH2 protein (Catalog#12588-H08B)
Species Reactivity
Human SerpinB1 / ELANH2
Source
Monoclonal Human Mouse IgG1
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinB1 / ELANH2 (rh SerpinB1 / ELANH2; Catalog#12588-H08B; P30740; Met1-Pro379). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SERPINB1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-EIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ELANH2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-27ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL57ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LEIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-M/NEIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MNEIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PI-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PI2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SERPINB1 Background Information
SerpinB1 is an endogenous inhibitor of serine proteases recognized for its anti-inflammatory and host-protective properties. Serum SerpinB1 levels are elevated in patients with type 2 diabetes compared with that in healthy subjects and are negatively correlated with serum LDL-C. serpinB1 as an endogenous protein that can potentially be harnessed to enhance functional beta cell mass in patients with diabetes.
Full Name
serpin peptidase inhibitor, clade B (ovalbumin), member 1