Anti-Cathepsin D Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Cathepsin D Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Cathepsin D magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Cathepsin D Magnetic Beads-IP Kit Product Description
The Anti-Cathepsin D magnetic Beads, conjugated with Anti-Cathepsin D antibody, are used for immuneprecipitation (IP) of Cathepsin D proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cathepsin D proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cathepsin D proteins are dissociated from the magnetic beads using an elution buffer. Anti-Cathepsin D Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Cathepsin D / CTSD protein (Catalog#12517-H08H)
Species Reactivity
Human Cathepsin D / CTSD
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Cathepsin D / CTSD (rh Cathepsin D / CTSD; Catalog#12517-H08H; P07339; Met 1-Leu 412). Cathepsin D / CTSD specific IgG was purified by Human Cathepsin D / CTSD affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Cathepsin D Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CLN10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CPSDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-130PALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Cathepsin D Background Information
Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.
References
Fusek M, et al. (2005) Dual role of cathepsin D: ligand and protease. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 149(1): 43-50. Minarowska A, et al. (2007) Regulatory role of cathepsin D in apoptosis. Folia Histochem Cytobiol. 45(3): 159-63. Zaidi N, et al. (2008) Cathepsin D: a cellular roadmap. Biochem Biophys Res Commun. 376(1): 5-9.
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