Anti-PARK7 / DJ-1 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-PARK7 / DJ-1 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PARK7 / DJ-1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PARK7 / DJ-1 Magnetic Beads-IP Kit Product Description
The Anti-PARK7 / DJ-1 magnetic Beads, conjugated with Anti-PARK7 / DJ-1 antibody, are used for immuneprecipitation (IP) of PARK7 / DJ-1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PARK7 / DJ-1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PARK7 / DJ-1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PARK7 / DJ-1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PARK7 / DJ-1 protein (Catalog#12484-H08E)
Species Reactivity
Human PARK7 / DJ-1
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PARK7 / DJ-1 (rh PARK7 / DJ-1; Catalog#12484-H08E; Q99497-1; Met1-Asp189).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PARK7 / DJ-1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DJ-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DJ1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-67pALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PARK7 / DJ-1 Background Information
Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke.
Full Name
parkinson protein 7
References
Takahashi K, et al. (2001). DJ-1 positively regulates the androgen receptor by impairing the binding of PIASx alpha to the receptor. J. Biol. Chem. (United States). 276 (40): 37556-63. Niki, Takeshi, et al. (2003). DJBP: a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex. Mol. Cancer Res. (United States). 1 (4): 247-61. Kahle PJ, et al. (2009) DJ-1 and prevention of oxidative stress in Parkinson's disease and other age-related disorders. Free Radic Biol Med. 47(10): 1354-61. Xu X, et al. (2010) The familial Parkinson's disease gene DJ-1 (PARK7) is expressed in red cells and plays a role in protection against oxidative damage. Blood Cells Mol Dis. 45(3): 227-32. Marcondes AM, et al. (2010) Identification of DJ-1/PARK-7 as a determinant of stroma-dependent and TNF-alpha-induced apoptosis in MDS using mass spectrometry and phosphopeptide analysis. Blood. 115(10): 1993-2002.