Anti-CD59 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD59 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD59 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD59 Magnetic Beads-IP Kit Product Description
The Anti-CD59 magnetic Beads, conjugated with Anti-CD59 antibody, are used for immuneprecipitation (IP) of CD59 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD59 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD59 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD59 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD59 protein (Catalog#12474-H08H)
Species Reactivity
Human CD59
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD59 (rh CD59; Catalog#12474-H08H; P13987-1; Met 1-Glu 101). CD59 specific IgG was purified by Human CD59 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD59 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-16.3A5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-1F5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EJ16ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EJ30ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EL32ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FLJ38134ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FLJ92039ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-G344ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HRF-20ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HRF20ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAC-IPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MACIFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MEM43ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGC2354ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIC11ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIN1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIN2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIN3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIRLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MSK21ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p18-20ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD59 Background Information
CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
References
Fletcher CM. et al., 1994, Structure. 2: 185-99. Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44. Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81. Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9. Picariello G. et al., 2008, Proteomics 8: 3833-47. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
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