Anti-PHPT1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PHPT1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PHPT1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PHPT1 Magnetic Beads-IP Kit Product Description
The Anti-PHPT1 magnetic Beads, conjugated with Anti-PHPT1 antibody, are used for immuneprecipitation (IP) of PHPT1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PHPT1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PHPT1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PHPT1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PHPT1 protein (Catalog#12473-H07E)
Species Reactivity
Human PHPT1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PHPT1 (rh PHPT1; Catalog#12473-H07E; Q9NRX4-1; Ala2-Tyr125). PHPT1 specific IgG was purified by Human PHPT1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PHPT1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CGI-202ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-132PALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSPC141ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PHP14ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PHPT1 Background Information
PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1 / PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1 / PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues.
Full Name
phosphohistidine phosphatase 1
References
Busam,R.D. et al., 2006, J Biol Chem. 281 (45):33830-4. Zhang,X.Q. et al., 2009, Ups J Med Sci.114 (2):65-72. Gong,W. et al., 2009, Biochem J. 418 (2):337-44. Chapin,L.J. et al., 2009, J Exp Bot. 60 (7):2179-90.
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