Anti-Adenylate Kinase 1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Adenylate Kinase 1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Adenylate Kinase 1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Adenylate Kinase 1 Magnetic Beads-IP Kit Product Description
The Anti-Adenylate Kinase 1 magnetic Beads, conjugated with Anti-Adenylate Kinase 1 antibody, are used for immuneprecipitation (IP) of Adenylate Kinase 1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Adenylate Kinase 1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Adenylate Kinase 1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Adenylate Kinase 1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human AK1 / Adenylate kinase 1 Protein (Catalog#12442-H07E)
Species Reactivity
Human AK1 / Adenylate kinase 1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human AK1 / Adenylate kinase 1 (rh AK1 / Adenylate kinase 1; Catalog#12442-H07E; AAH01116.1; Met1-Lys194). AK1 / Adenylate kinase 1 specific IgG was purified by Human AK1 / Adenylate kinase 1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Adenylate Kinase 1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HTL-S-58jALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Adenylate Kinase 1 Background Information
The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. Three other biomarkers (AK1, CLIC1, and SOD1) were significantly increased in both adenoma and early colorectal cancer patient plasma samples and in plasma from colorectal cancer mouse models at preclinical stages compared with controls.Which can contribute to the detection of early-stage colorectal cancer and adenomas.
Full Name
adenylate kinase 1
References
Lu Q, et al. (1996) Adenylate kinase complements nucleoside diphosphate kinase deficiency in nucleotide metabolism. Proc Natl Acad Sci. 93 (12): 5720-5. Dzeja P, et al. (2009) Adenylate kinase and AMP signaling networks: Metabolic monitoring, signal communication and body energy sensing. Int J Mol Sci. 10 (4): 1729-72. Lagresle-Peyrou C, et al. (2008) Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nature Genetics. 41: 106 - 11.
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