Anti-RhoA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-RhoA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-RhoA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-RhoA Magnetic Beads-IP Kit Product Description
The Anti-RhoA magnetic Beads, conjugated with Anti-RhoA antibody, are used for immuneprecipitation (IP) of RhoA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing RhoA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound RhoA proteins are dissociated from the magnetic beads using an elution buffer. Anti-RhoA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human RhoA protein (Catalog#12441-H07B)
Species Reactivity
Human RhoA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human RhoA (rh RhoA; Catalog#12441-H07B; P61586-1; Met 1-Leu 193). RhoA specific IgG was purified by Human RhoA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-RhoA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ARH12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ARHAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RHO12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RHOH12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
RhoA Background Information
Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.
Full Name
ras homolog family member A
References
Kiss C, et al.,1997, Cytogenet. Cell Genet. 79 (3-4): 228-30. Anastasiadis,P.Z. et al., 2000, Nat Cell Biol. 2 (9):637-44. Yiu G, et al.,2006, Nat. Rev. Neurosci. 7 (8): 617-27. Wang,HR. et al., 2006, Methods Enzymol. 406 :437-47. Heo,J. et al., 2006, Biochemistry. 45 (48):14481-9.
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