Anti-C1D Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-C1D Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-C1D magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-C1D Magnetic Beads-IP Kit Product Description
The Anti-C1D magnetic Beads, conjugated with Anti-C1D antibody, are used for immuneprecipitation (IP) of C1D proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing C1D proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound C1D proteins are dissociated from the magnetic beads using an elution buffer. Anti-C1D Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human C1D protein (Catalog#12432-H09E)
Species Reactivity
Human C1D
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human C1D (rh C1D; Catalog#12432-H09E; Q13901; Met 1-Ser 141). C1D specific IgG was purified by Human C1D affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-C1D Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-hC1DALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LRP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Rrp47ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SUN-CoRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SUNCORALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
C1D Background Information
C1D nuclear receptor corepressor belongs to the C1D family. It is a DNA binding and apoptosis-inducing protein.C1D nuclear receptor corepressorinteracts with TSNAX and DNA-PKcs. It acts as a corepressor for the thyroid hormone receptor. It is thought that C1D nuclear receptor corepressor regulates TRAX/Translin complex formation. It is expressed in kidney, heart, brain, spleen, lung, testis, liver and small intestine. It plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. It potentiates transcriptional repression by NR1D1 and THRB. C1D nuclear receptor corepressorcan activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. It also can induce apoptosis in a p53/TP53 dependent manner.
Full Name
C1D nuclear receptor corepressor
References
Schilders G, et al. (2007) C1D is a major autoantibody target in patients with the polymyositis-scleroderma overlap syndrome. Arthritis Rheum.56(7):2449-54. Schilders G, et al. (2007) C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing. Nucleic Acids Res. 35(8):2564-72. Erdemir T, et al. (2002) DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX). J Cell Sci. 115(Pt 1):207-16.
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