Anti-MICA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-MICA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-MICA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-MICA Magnetic Beads-IP Kit Product Description
The Anti-MICA magnetic Beads, conjugated with Anti-MICA antibody, are used for immuneprecipitation (IP) of MICA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MICA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MICA proteins are dissociated from the magnetic beads using an elution buffer. Anti-MICA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human MICA protein (Catalog#12302-H08H)
Species Reactivity
Human MICA
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human MICA (rh MICA; Catalog#12302-H08H; AAH16929.1; Met 1-Gln 308). MICA specific IgG was purified by Human MICA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-MICA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DAMA-345G11.2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FLJ36918ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FLJ60820ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGC111087ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGC21250ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIC-AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PERB11.1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
MICA Background Information
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.
Full Name
MHC class I polypeptide-related sequence A
References
Choy MK, et al. (2010) MICA polymorphism: biology and importance in immunity and disease. Trends Mol Med. 16(3): 97-106. Li J, et al. (2005) Distinct pattern of human Vdelta1 gammadelta T cells recognizing MICA. Cell Mol Immunol. 2(4): 253-8. Mangham DC, et al. (2000) MICA-a highly sensitive and avidin-free immunohistochemical detection system. Adv Anat Pathol. 7(6): 360-4. Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428): 727-9. Groh V, et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 279: 1737-40.
新浪微博
腾讯微博
QQ空间
客服
