Anti-CA5B Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CA5B Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CA5B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CA5B Magnetic Beads-IP Kit Product Description
The Anti-CA5B magnetic Beads, conjugated with Anti-CA5B antibody, are used for immuneprecipitation (IP) of CA5B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CA5B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CA5B proteins are dissociated from the magnetic beads using an elution buffer. Anti-CA5B Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Carbonic Anhydrase VB / CA5B Protein (Catalog#12285-H08E)
Species Reactivity
Human Carbonic Anhydrase VB /
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VB / CA5B (rh Carbonic Anhydrase VB / CA5B; Catalog#12285-H08E; Q9Y2D0; Cys34-Pro317). Carbonic Anhydrase VB / CA5B specific IgG was purified by Human Carbonic Anhydrase VB / CA5B affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CA5B Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CA-VBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGC39962ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CA5B Background Information
Carbonic anhydrase 5B, also known as carbonate dehydratase VB, carbonic anhydrase VB, CA-VB and CA5B, is a member of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart, pancreas, kidney, placenta, lung, and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B / CA-VB is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin and Foscarnet (phosphonoformate trisodium salt).
Full Name
carbonic anhydrase VB, mitochondrial
References
Fujikawa-Adachi K, et al.,1999, J Biol Chem. 274 (30): 21228-33. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. Temperini C.et al., 2006, Chemistry 12: 7057-66. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222. Maresca, A.et al., 2009, J. Am. Chem. Soc. 131:3057-3062.
新浪微博
腾讯微博
QQ空间
客服
