Anti-CD82 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD82 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD82 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD82 Magnetic Beads-IP Kit Product Description
The Anti-CD82 magnetic Beads, conjugated with Anti-CD82 antibody, are used for immuneprecipitation (IP) of CD82 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD82 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD82 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD82 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD82 / KAI-1 protein (Catalog#12275-H08H)
Species Reactivity
Human CD82 / KAI-1
Source
Polyclonal Human Rabbit IgG
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD82 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-4F9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-C33ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GR15ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IA4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-KAI-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-KAI1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-R2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SAR2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ST6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TSPAN27ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD82 Background Information
CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.
References
Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30. Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94. Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70. Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
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