Anti-CLIC4 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CLIC4 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CLIC4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CLIC4 Magnetic Beads-IP Kit Product Description
The Anti-CLIC4 magnetic Beads, conjugated with Anti-CLIC4 antibody, are used for immuneprecipitation (IP) of CLIC4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CLIC4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CLIC4 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CLIC4 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CLIC4 protein (Catalog#12271-H07E)
Species Reactivity
Human CLIC4
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CLIC4 (rh CLIC4; Catalog#12271-H07E; Q9Y696-1; Ala 2-Lys 253). CLIC4 specific IgG was purified by Human CLIC4 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CLIC4 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CLIC4LALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-H1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-huH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MTCLICALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p64H1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CLIC4 Background Information
Chloride intracellular channel protein 4, also known as Intracellular chloride ion channel protein p64H1 and CLIC4, is a member of the chloride channel CLIC family. It contains one GST C-terminal domain. CLIC4 is a member of a family of intracellular chloride channels. It is regulated by p53, c-Myc, and tumor necrosis factor-alpha. CLIC4 is detected in epithelial cells from colon, esophagus and kidney (at protein level). CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. CLIC4 could promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Expression of CLIC4 is prominent in heart, kidney, placenta and skeletal muscle. Overexpression of CLIC4 in cancer cells inhibits tumor growth. Conversely, overexpression of CLIC4 in tumor stromal cells stimulates tumor growth. Thus, CLIC4 participates in normal and pathological processes and may serve as a useful target for therapies in disturbances of homeostasis and neoplastic transformation. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion, generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth.
Full Name
chloride intracellular channel 4
References
Ronnov-Jessen L., et al., 2002, Am. J. Pathol. 161:471-480. Berryman M.A., et al., 2003, Cell Motil. Cytoskeleton 56:159-172. Suh,K.S. et al., 2005, J Investig Dermatol Symp Proc. 10 (2):105-9. Suh,K.S. et al., 2007, Mol Carcinog.46 (8):599-604. Suh,K.S. et al., 2007, J Cell Sci 120 (Pt 15):2631-40. Suh,K.S. et al., 2007, Clin Cancer Res 13 (1):121-31.
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