Anti-SECTM1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SECTM1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SECTM1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SECTM1 Magnetic Beads-IP Kit Product Description
The Anti-SECTM1 magnetic Beads, conjugated with Anti-SECTM1 antibody, are used for immuneprecipitation (IP) of SECTM1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SECTM1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SECTM1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SECTM1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SECTM1 Protein (Catalog#12269-H08H)
Species Reactivity
Human SECTM1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SECTM1 (rh SECTM1; Catalog#10045-H08H; NP_001618.2; Met 1-Ala 526). SECTM1 specific IgG was purified by Human SECTM1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SECTM1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-K12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SECTM1 Background Information
Secreted and transmembrane 1 (SECTM1), also known as K12, is a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein of SECTM family. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The human K12 protein has been shown to be primarily expressed in spleen, prostate, testis, small intestine, and in peripheral blood leukocytes. The K12 protein is expressed on the cell surface in such small amounts as to preclude detection. Alternatively, it may be that K12 on the cell surface is rapidly cleaved to generate a soluble K12 protein. Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. May be involved in thymocyte signaling. It had been suggested a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis. In addition, as a putative natural CD7 ligand, SECTM1/K12 may be responsible for the costimulatory role it plays in T cell activation.
Full Name
secreted and transmembrane 1
References
Leta E, et al. (1995) Production and characterization of the extracellular domain of human CD7 antigen: further evidence that CD7 has a role in T cell signaling. Cell Immunol. 165(1): 101-9. Slentz-Kesler KA, et al. (1998) Identification and characterization of K12 (SECTM1), a novel human gene that encodes a Golgi-associated protein with transmembrane and secreted isoforms. Genomics. 47(3): 327-40. Lyman SD, et al. (2000) Identification of CD7 as a cognate of the human K12 (SECTM1) protein. J Biol Chem. 275(5): 3431-7. Lam GK, et al. (2005) Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma. J Clin Immunol. 25(1): 41-9.
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