Anti-CD164 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD164 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD164 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD164 Magnetic Beads-IP Kit Product Description
The Anti-CD164 magnetic Beads, conjugated with Anti-CD164 antibody, are used for immuneprecipitation (IP) of CD164 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD164 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD164 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD164 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD164 / Endolyn protein (Catalog#12260-H08H)
Species Reactivity
Human CD164 / Endolyn
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD164 / Endolyn (rh CD164 / Endolyn; Catalog#12260-H08H; Q04900-1; Met1-Asp162). CD164 / Endolyn specific IgG was purified by Human CD164 / Endolyn affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD164 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-endolynALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MGC-24ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MUC-24ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD164 Background Information
Sialomucin core protein 24 also known as endolyn or CD164 (cluster of differentiation 164) is a novel 8- to 9-kD mucin-like molecule expressed by human CD34+ hematopoietic progenitor cells. Isoform 1 and isoform 3 of CD164 are expressed in hematopoietic and non-hematopoietic tissues. Isoform 1 is expressed by prostate cancer tumors and prostate cancer cell lines. The expression is greater in bone metastases than in primary tumors. Expression in osseous metastasis is greater than that in soft tissue metastasis. Isoform 2 of CD164 is expressed in the small intestine, colon, lung, thyroid and in colorectal and pancreatic adenocarcinoma. Isoform 4 is expressed by both hematopoietic progenitor cells and bone marrow stromal cells. CD164 belongs to the CD164 family. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. CD164 may play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. CD164 promotes myogenesis by enhancing CXCR4-dependent cell motility. This protein positively regulates myoblast migration and promotes myoblast fusion into myotubes. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to bone marrow stroma and by negatively regulating CD34+hematopoietic progenitor cell growth.
Full Name
CD164 molecule, sialomucin
References
McGuckin CP, et al. (2003) Colocalization analysis of sialomucins CD34 and CD164. Stem Cells. 21(2): 162-70. Doyonnas R, et al. (2000) CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 165(2): 840-51. Zannettino AC, et al. (1998) The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 92(8): 2613-28.
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