Anti-CD46 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD46 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD46 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD46 Magnetic Beads-IP Kit Product Description
The Anti-CD46 magnetic Beads, conjugated with Anti-CD46 antibody, are used for immuneprecipitation (IP) of CD46 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD46 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD46 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD46 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD46 protein (Catalog#12239-H08H)
Species Reactivity
Human CD46
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). CD46 specific IgG was purified by Human CD46 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD46 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AHUS2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIC10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TLXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TRA2.10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD46 Background Information
CD46, also known as Membrane Cofactor Protein (MCP), is a complement regulatory protein. CD46 is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. Human CD46 shares 5% amino acid sequence identity with mouse and rat CD46. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation. CD46 is implicated in the development and/or progression of selected cancer types.
Full Name
CD46 molecule, complement regulatory protein
References
Lublin D.M., et al.,(1988), Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP). Evidence for inclusion in the multigene family of complement-regulatory proteins. J. Exp. Med. 168:181-194. Purcell D.F., et al., (1991), Alternatively spliced RNAs encode several isoforms of CD46 (MCP), a regulator of complement activation.Immunogenetics 33:335-344. Post T.W., et al.,(1991), Membrane cofactor protein of the complement system: alternative splicing of serine/threonine/proline-rich exons and cytoplasmic tails produces multiple isoforms that correlate with protein phenotype.J. Exp. Med. 174:93-102.
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