Anti-CD98 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD98 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD98 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD98 Magnetic Beads-IP Kit Product Description
The Anti-CD98 magnetic Beads, conjugated with Anti-CD98 antibody, are used for immuneprecipitation (IP) of CD98 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD98 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD98 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD98 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD98 / SLC3A2 protein (Catalog#12206-H07H)
Species Reactivity
Human CD98 / SLC3A2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CD98 / SLC3A2 (rh CD98 / SLC3A2; Catalog#12206-H07H; P08195-1; Arg 206-Ala 630). CD98 / SLC3A2 specific IgG was purified by Human CD98 / SLC3A2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD98 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-4F2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-4F2HCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-4T2HCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD98ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CD98HCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MDU1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NACAEALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD98 Background Information
4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family. SLC3A2 / MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2 / MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2 / MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2 / MDU1 is required for the function of light chain amino-acid transporters. It involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2 / MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2 / MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2 / MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2 / MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.
Full Name
solute carrier family 3 (amino acid transporter heavy chain), member 2
References
Torrents D. et al., 1998, J Biol Chem. 273: 32437-45. Pfeiffer R. et al., 1999, EMBO J. 18: 49-57. Broeer A. et al., 2000, Biochem J. 349: 787-95. Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302. Broeer A. et al., 2001, Biochem J. 355: 725-31. Fort J. et al., 2007, J Biol Chem. 282: 31444-52. Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.
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