Anti-Glyoxalase II Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Glyoxalase II Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Glyoxalase II magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Glyoxalase II Magnetic Beads-IP Kit Product Description
The Anti-Glyoxalase II magnetic Beads, conjugated with Anti-Glyoxalase II antibody, are used for immuneprecipitation (IP) of Glyoxalase II proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Glyoxalase II proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Glyoxalase II proteins are dissociated from the magnetic beads using an elution buffer. Anti-Glyoxalase II Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human HAGH / GLO2 / Glyoxalase II protein (Catalog#12146-H08E)
Species Reactivity
Human HAGH / GLO2 / Glyoxalase
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human HAGH / GLO2 / Glyoxalase II (rh HAGH / GLO2 / Glyoxalase II; Catalog#12146-H08E; Q16775-2; Met 1-Asp 260). HAGH / GLO2 / Glyoxalase II specific IgG was purified by Human HAGH / GLO2 / Glyoxalase II affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Glyoxalase II Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GLO2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GLX2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GLXIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HAGH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Glyoxalase II Background Information
The human and rodent forms of glyoxalase II (Hydroxyacylglutathione hydrolase, HAGH) can readily be separated by starch gel electrophoretic procedures.
Full Name
hydroxyacylglutathione hydrolase
References
Mulley JC, et al. (1987). New regional localisations for HAGH and PGP on human chromosome 16. Hum Genet 74 (4): 423-4. Rulli A, et al. (2001). Expression of glyoxalase I and II in normal and breast cancer tissues. Breast Cancer Res. Treat. 66 (1): 67-72. Cordell PA, et al. (2004). The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II. J Biol Chem. 279 (27): 28653-61.
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