Anti-Marapsin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Marapsin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Marapsin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Marapsin Magnetic Beads-IP Kit Product Description
The Anti-Marapsin magnetic Beads, conjugated with Anti-Marapsin antibody, are used for immuneprecipitation (IP) of Marapsin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Marapsin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Marapsin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Marapsin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Pancreasin / Marapsin / PRSS27 protein (Catalog#12116-H08H)
Species Reactivity
Human Pancreasin / Marapsin /
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Pancreasin / Marapsin / PRSS27 (rh Pancreasin / Marapsin / PRSS27; Catalog#12116-H08H; Q9BQR3; Met1-Lys290).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Marapsin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CAPH2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MPNALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UNQ1884/PRO4327ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Marapsin Background Information
The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), which is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
Full Name
protease, serine 27
References
Bhagwandin VJ, et al. (2003) Structure and activity of human pancreasin, a novel tryptic serine peptidase expressed primarily by the pancreas. J Biol Chem. 278(5): 3363-71. Li W, et al. (2009) The serine protease marapsin is expressed in stratified squamous epithelia and is up-regulated in the hyperproliferative epidermis of psoriasis and regenerating wounds. J Biol Chem. 284(1): 218-28.
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