Anti-FH Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-FH Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FH magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FH Magnetic Beads-IP Kit Product Description
The Anti-FH magnetic Beads, conjugated with Anti-FH antibody, are used for immuneprecipitation (IP) of FH proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FH proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FH proteins are dissociated from the magnetic beads using an elution buffer. Anti-FH Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Fumarate Hydratase / FH protein (Catalog#12115-H08E)
Species Reactivity
Human Fumarate Hydratase / FH
Source
Monoclonal Human Mouse IgG1
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fumarate Hydratase / FH (rh Fumarate Hydratase / FH; Catalog#12115-H08E; P07954-1; Ala 45-Lys 510). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FH Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-FMRDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HLRCCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LRCCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCUL1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FH Background Information
Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event.
Full Name
fumarate hydratase
References
King A, et al. (2005) Succinate dehydrogenase and fumarate hydratase: linking mitochondrial dysfunction and cancer. Oncogene. 25(34): 4675-82. Alam NA, et al. (2003) Genetic and functional analyses of FH mutations in multiple cutaneous and uterine leiomyomatosis, hereditary leiomyomatosis and renal cancer, and fumarate hydratase deficiency. Hum Mol Genet.12(11): 1241-52. Pollard PJ, et al. (2003) The TCA cycle and tumorigenesis: the examples of fumarate hydratase and succinate dehydrogenase. Ann Med. 35(8): 632-9.
新浪微博
腾讯微博
QQ空间
客服
