Anti-FABP4 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-FABP4 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FABP4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FABP4 Magnetic Beads-IP Kit Product Description
The Anti-FABP4 magnetic Beads, conjugated with Anti-FABP4 antibody, are used for immuneprecipitation (IP) of FABP4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FABP4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FABP4 proteins are dissociated from the magnetic beads using an elution buffer. Anti-FABP4 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human FABP4 / ALBP / A-FABP protein (Catalog#12109-H07E)
Species Reactivity
Human FABP4 / ALBP / A-FABP
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human FABP4 / ALBP / A-FABP (rh FABP4 / ALBP / A-FABP; Catalog#12109-H07E; P15090; Cys2-Ala 132, 29Ala /Thr ). FABP4 / ALBP / A-FABP specific IgG was purified by Human FABP4 / ALBP / A-FABP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FABP4 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-A-FABPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AFABPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ALBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-aP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-104ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FABP4 Background Information
Fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. In familial combined hyperlipidemia (FCHL), FABP4 correlated to body mass index (BMI), waist circumference and homeostasis model assessment (HOMA) index.FABP4 levels were associated with triglyceride-rich lipoproteins. In humans serum FABP4 levels correlate significantly with features of PCOS. It appears to be a determinant of atherogenic dyslipidemia. FABP4 pathway mediates the sebaceous gland hyperplasia in keratinocyte-specific Pten-null mice. FABP4 concentration significantly increased with an increasing of MS features and was strongly correlated with body-mass index, triglycerides, HDL-cholesterol concentrations and blood pressure. Patients in the highest quartile of FABP4 presented a six-fold increased odds ratio for MS and a three-fold increased odds for LD, adjusted by age, sex, body-mass index and the antiretroviral therapy. FABP4 is a strong plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.
Full Name
fatty acid binding protein 4, adipocyte
References
van Dongen,M.J. et al., 2002, J Am Chem Soc. 124 (40): 11874-80. Coll, B. et al., 2008, Atherosclerosis 199 (1):147-53. Hoashi,S. et al., 2008, BMC Genet. 9 :84. Cai,H. et al., 2010, Bioorg Med Chem Lett 20 (12):3675-9.
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