Anti-Nucleoside phosphorylase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Nucleoside phosphorylase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Nucleoside phosphorylase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Nucleoside phosphorylase Magnetic Beads-IP Kit Product Description
The Anti-Nucleoside phosphorylase magnetic Beads, conjugated with Anti-Nucleoside phosphorylase antibody, are used for immuneprecipitation (IP) of Nucleoside phosphorylase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Nucleoside phosphorylase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Nucleoside phosphorylase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Nucleoside phosphorylase Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Nucleoside phosphorylase Antibody(
12097-T52)
Immunogen
Recombinant Human PNP protein (Catalog#12097-H08E)
Species Reactivity
Human PNP
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PNP (rh PNP; Catalog#12097-H08E; P00491; Met1-Ser289). PNP specific IgG was purified by Human PNP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Nucleoside phosphorylase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-NPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PRO1837ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PUNPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Nucleoside phosphorylase Background Information
Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells.Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency.Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.
Full Name
purine nucleoside phosphorylase
References
de Azevedo,W.F. et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72. Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (Pt 7): 856-62. Perera,G.K. et al., 2005,Clin Exp Dermatol. 30 (1):27-9.
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