Anti-NRAS Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-NRAS Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-NRAS magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-NRAS Magnetic Beads-IP Kit Product Description
The Anti-NRAS magnetic Beads, conjugated with Anti-NRAS antibody, are used for immuneprecipitation (IP) of NRAS proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing NRAS proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound NRAS proteins are dissociated from the magnetic beads using an elution buffer. Anti-NRAS Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human NRAS / N-Ras protein (Catalog#12073-H07E)
Species Reactivity
Human NRAS / N-Ras
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human NRAS / N-Ras (rh NRAS / N-Ras; Catalog#12073-H07E; P01111; Met1-Cys186). NRAS / N-Ras specific IgG was purified by Human NRAS / N-Ras affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-NRAS Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ALPS4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CMNSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-N-rasALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NCMSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NRAS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NS6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
NRAS Background Information
NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL6, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 4 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
neuroblastoma RAS viral (v-ras) oncogene homolog
References
Marshall CJ. et al., 1982, Nature. 299 (5879): 171-3. Hall Alan. et al., 1983, Nature. 303 (5916): 396-400. McCormick F. 1996, Mol Reprod Dev. 42 (4): 500-6.
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