Anti-NQO1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-NQO1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-NQO1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-NQO1 Magnetic Beads-IP Kit Product Description
The Anti-NQO1 magnetic Beads, conjugated with Anti-NQO1 antibody, are used for immuneprecipitation (IP) of NQO1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing NQO1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound NQO1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-NQO1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human NQO1 Protein (Catalog#12046-H07E)
Species Reactivity
Human NQO1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human NQO1 (rh NQO1; Catalog#12046-H07E; P15559-1; Met1-Lys274). NQO1 specific IgG was purified by Human NQO1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-NQO1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DHQUALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DIA4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DTDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NMOR1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NMORIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-QR1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
NQO1 Background Information
NQO1 gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. NQO1 forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in NQO1 gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recent pharmacological research suggests feasibility of genotype-directed redox chemotherapeutic intervention targeting NQO1 breast cancer, a common missense genotype encoding a functionally impaired NQO1 protein.
Full Name
NAD(P)H dehydrogenase, quinone 1
References
Ross David. et al., 2002, J Biol Chem. 277 (16): 14060-7. Cabello CM. et al., 2010, Free Radic Res. 45 (3): 276-92. Adesnik M. et al., 1988, J Biol Chem. 263 (27): 13572-8.
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