Anti-CAMKI Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CAMKI Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CAMKI magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CAMKI Magnetic Beads-IP Kit Product Description
The Anti-CAMKI magnetic Beads, conjugated with Anti-CAMKI antibody, are used for immuneprecipitation (IP) of CAMKI proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CAMKI proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CAMKI proteins are dissociated from the magnetic beads using an elution buffer. Anti-CAMKI Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CAMKI / CAMK1 protein (Catalog#11932-HNCE)
Species Reactivity
Human CAMKI / CAMK1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CAMKI / CAMK1 (rh CAMKI / CAMK1; Catalog#11932-HNCE; NP_003647.1; Leu 2-Leu 370). CAMKI / CAMK1 specific IgG was purified by Human CAMKI / CAMK1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CAMKI Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CAMKIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CAMKI Background Information
Calcium/calmodulin-dependent protein kinase or CaM kinases are serine/threonine-specific protein kinases that are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. In neurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction of long-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of the protein kinase superfamily and CaMK subfamily. It contains one protein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.
Full Name
calcium/calmodulin-dependent protein kinase I
References
Lisman, JE. et al., 1985, Proc Natl Acad Sci USA. 82 (9): 3055-7. Bergamaschi, A. et al., 2008, Mol Oncol. 2 (4): 327-39. White RB. et al., 2008, Physiological genomics, 33 (1): 41-9. Schleinitz, D. et al., 2010, Horm Metab Res. 42 (1): 14-22.
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