Anti-CD19 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD19 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD19 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD19 Magnetic Beads-IP Kit Product Description
The Anti-CD19 magnetic Beads, conjugated with Anti-CD19 antibody, are used for immuneprecipitation (IP) of CD19 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD19 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD19 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD19 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD19 protein (Catalog#11880-H08H)
Species Reactivity
Human CD19
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391; Met1-Lys291).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD19 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-B4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CVID3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD19 Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell coreceptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.
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