Anti-IL17F Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-IL17F Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-IL17F magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-IL17F Magnetic Beads-IP Kit Product Description
The Anti-IL17F magnetic Beads, conjugated with Anti-IL17F antibody, are used for immuneprecipitation (IP) of IL17F proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IL17F proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IL17F proteins are dissociated from the magnetic beads using an elution buffer. Anti-IL17F Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human IL-17F protein (Catalog#11855-H07H)
Species Reactivity
Human IL-17F
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human IL-17F (rh IL-17F; Catalog#11855-H07H; NP_443104.1; Arg31-Gln163). IL-17F specific IgG was purified by Human IL-17F affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-IL17F Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CANDF6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IL-17FALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ML-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ML1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
IL17F Background Information
Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells, and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also function in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Full Name
interleukin 17F
References
et al..
Starnes T, et al.. (2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. J Immunol. 167(8): 4137-40. Hymowitz SG, et al.. (2001) IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding. EMBO J. 20(19): 5332-41. McAllister F, et al.. (2005) Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in cystic fibrosis. J Immunol. 175(1): 404-12.