Anti-ATP citrate lyase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ATP citrate lyase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ATP citrate lyase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ATP citrate lyase Magnetic Beads-IP Kit Product Description
The Anti-ATP citrate lyase magnetic Beads, conjugated with Anti-ATP citrate lyase antibody, are used for immuneprecipitation (IP) of ATP citrate lyase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ATP citrate lyase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ATP citrate lyase proteins are dissociated from the magnetic beads using an elution buffer. Anti-ATP citrate lyase Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ACLY / ATP Protein (Catalog#11769-H07B)
Species Reactivity
Human ACLY / ATP
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ACLY / ATP (rh ACLY / ATP; Catalog#11769-H07B; P53396; Met1-Met1101). ACLY / ATP specific IgG was purified by Human ACLY / ATP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ATP citrate lyase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ACLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ATPCLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CLATPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ATP citrate lyase Background Information
ATP citrate lyase, also known as Acly or Acl, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is composed of two polymer chains which are polypeptides in human. ATP citrate lyase is responsible for catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In vivo, it reduces tumor growth leading to a cytostatic effect and induces differentiation.
Full Name
ATP citrate lyase
References
Kim W, et al. (2006) Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. J Bacteriol. 188 (18) : 6544-52. Ki SW, et al. (2000) Radicicol binds and inhibits mammalian ATP citrate lyase. J Biol Chem. 275 (50) : 39231-6. Schneider K, et al. (2000) Biosynthesis of the prosthetic group of citrate lyase. Biochemistry. 39 (31) : 9438-50.
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