Anti-HSP70 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-HSP70 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-HSP70 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-HSP70 Magnetic Beads-IP Kit Product Description
The Anti-HSP70 magnetic Beads, conjugated with Anti-HSP70 antibody, are used for immuneprecipitation (IP) of HSP70 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing HSP70 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound HSP70 proteins are dissociated from the magnetic beads using an elution buffer. Anti-HSP70 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human HSP70 / HSPA1A protein (Catalog#11660-H07B)
Species Reactivity
Human HSP70 / HSPA1A
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human HSP70 / HSPA1A (rh HSP70 / HSPA1A; Catalog#11660-H07B; P08107; Ala2-Asp641). HSP70 / HSPA1A specific IgG was purified by Human HSP70 / HSPA1A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-HSP70 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HEL-S-103ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSP70-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSP70-1AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSP70IALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSP72ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSPA1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
HSP70 Background Information
HSPA1A is a member of the Hsp7 protein family. The 7 kilodalton heat shock proteins (Hsp7s) are a family of ubiquitously expressed heat shock proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature shock or other stress stimuli, HSP are synthesized intracellularly, which may protect cells from protein denaturation or from death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates presentation of antigen peptide to T cells. Molecular chaperones of the Hsp7 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp7 chaperones are ATP dependent: binding and hydrolysis of ATP regulates their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp7 chaperones use the same mechanism of ATP-driven polypeptide binding and release.
Full Name
heat shock 70kDa protein 1A
References
Heck TG, et al. (2011) HSP70 expression: does it a novel fatigue signalling factor from immune system to the brain Cell Biochem Funct. 29 (3): 215-26. Chen T, et al. (2010) Stress for maintaining memory: HSP70 as a mobile messenger for innate and adaptive immunity. Eur J Immunol. 40 (6): 1541-4. Young JC. (2010) Mechanisms of the Hsp70 chaperone system. Biochem Cell Biol. 88 (2): 291-300.
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