Anti-SULT1A1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SULT1A1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SULT1A1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SULT1A1 Magnetic Beads-IP Kit Product Description
The Anti-SULT1A1 magnetic Beads, conjugated with Anti-SULT1A1 antibody, are used for immuneprecipitation (IP) of SULT1A1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SULT1A1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SULT1A1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SULT1A1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SULT1A1 protein (Catalog#11658-H07E)
Species Reactivity
Human SULT1A1
Source
Monoclonal Human Mouse IgG1
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT1A1 (rh SULT1A1; Catalog#11658-H07E; P50225-1; Glu 2-Leu 295). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SULT1A1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HAST1/HAST2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P-PSTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PSTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ST1A1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ST1A3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-STPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-STP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TSPST1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SULT1A1 Background Information
Sulfate conjugation catalyzed by cytosolic sulfotransferase (SULT) enzymes. The SULTs are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Particularly SULT1A1 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), a member of the sulfotransferase 1 subfamily, which is a major pathway for drug metabolism in humans. Humans have at least 1 functional SULT genes. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences. An Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer). Furthermore, the polymorphism of the SULT1A1 may be closely associated with breast cancer.
Full Name
sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
References
Coughtrie MW. 2002. Sulfation through the looking glass--recent advances in sulfotransferase research for the curious. Pharmacogenomics J. 2(5): 297-308. Klaassen CD, et al. (1998) Regulation of sulfotransferase mRNA expression in male and female rats of various ages. Chem Biol Interact. 109(1-3): 299-313. Glatt H. (2000) Sulfotransferases in the bioactivation of xenobiotics. Chem Biol Interact. 129(1-2): 141-70. Glatt H, et al. (2004) Pharmacogenetics of soluble sulfotransferases (SULTs). Naunyn Schmiedebergs Arch Pharmacol. 369(1): 55-68. Hebbring SJ, et al. (2008) Sulfotransferase gene copy number variation: pharmacogenetics and function. Cytogenet Genome Res. 123(1-4): 205-10.
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